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34 | 34 | "* **Assess read quality with FastQC:** Understand how to use FastQC to evaluate the quality of reads and identify potential biases or issues.\n",
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35 | 35 | "* **Generate a consolidated QC report with MultiQC:** Learn to use MultiQC to combine FastQC results and generate an overview of quality metrics across multiple samples.\n",
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36 | 36 | "* **Index a transcriptome with Salmon:** Understand the purpose of indexing and learn how to use Salmon to create an index of the reference transcriptome for efficient read mapping.\n",
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37 |
| - "* **Map reads to a transcriptome and quantify expression with Salmon:** Learn how to use Salmon to map reads to transcripts and quantify gene expression levels." |
| 37 | + "* **Map reads to a transcriptome and quantify expression with Salmon:** Learn how to use Salmon to map reads to transcripts and quantify gene expression levels.\n", |
| 38 | + "\n", |
| 39 | + "<div class=\"alert alert-block alert-success\">\n", |
| 40 | + " <i class=\"fa fa-hand-paper-o\" aria-hidden=\"true\"></i>\n", |
| 41 | + " <b>Tip: </b> If you're having trouble with any part of this tutorial, feel free to leverage AWS Bedrock (Amazon's advanced generative AI tool) at the bottom of this module.\n", |
| 42 | + "</div> " |
38 | 43 | ]
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39 | 44 | },
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40 | 45 | {
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|
415 | 420 | "\n",
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416 | 421 | "Remember to move to the next notebook or shut down your instance if you are finished."
|
417 | 422 | ]
|
| 423 | + }, |
| 424 | + { |
| 425 | + "cell_type": "markdown", |
| 426 | + "metadata": {}, |
| 427 | + "source": [ |
| 428 | + "## Bedrock (Optional)\n", |
| 429 | + "--------\n", |
| 430 | + "\n", |
| 431 | + "If you're having trouble with this submodule (or others within this tutorial), feel free to leverage Bedrock by running the cell below. AWS Bedrock is a fully managed service that simplifies building and scaling generative AI applications. It provides access to various foundation models (FMs) from Amazon and other AI companies.\n", |
| 432 | + "\n", |
| 433 | + "Before being able to use the chatbot you must request **Llama 3 8B Instruct** model access through AWS Bedrock. In order to do this follow the instructions to request model access provided in [AWS Bedrock Intro Notebook](https://github.com/STRIDES/NIHCloudLabAWS/blob/main/notebooks/GenAI/AWS_Bedrock_Intro.ipynb). After requesting the Llama 3 8B Instruct access it should only take a minute to get approved. While waiting for model approval attach the **AmazonBedrockFullAccess** permission to your notebook service role. Once approved run the following code cell to use the model within the notebook. " |
| 434 | + ] |
| 435 | + }, |
| 436 | + { |
| 437 | + "cell_type": "code", |
| 438 | + "execution_count": null, |
| 439 | + "metadata": {}, |
| 440 | + "outputs": [], |
| 441 | + "source": [ |
| 442 | + "# Ensure you have the necessary libraries installed\n", |
| 443 | + "!pip install -q ipywidgets\n", |
| 444 | + "import sys\n", |
| 445 | + "import os\n", |
| 446 | + "util_path = os.path.join(os.getcwd(), 'util')\n", |
| 447 | + "if util_path not in sys.path:\n", |
| 448 | + " sys.path.append(util_path)\n", |
| 449 | + "\n", |
| 450 | + "# Import the display_widgets function from your Python file\n", |
| 451 | + "from genai import display_widgets\n", |
| 452 | + "\n", |
| 453 | + "# Call the function to display the widgets\n", |
| 454 | + "display_widgets()" |
| 455 | + ] |
418 | 456 | }
|
419 | 457 | ],
|
420 | 458 | "metadata": {
|
| 459 | + "kernelspec": { |
| 460 | + "display_name": "conda_python3", |
| 461 | + "language": "python", |
| 462 | + "name": "conda_python3" |
| 463 | + }, |
421 | 464 | "language_info": {
|
422 |
| - "name": "python" |
| 465 | + "codemirror_mode": { |
| 466 | + "name": "ipython", |
| 467 | + "version": 3 |
| 468 | + }, |
| 469 | + "file_extension": ".py", |
| 470 | + "mimetype": "text/x-python", |
| 471 | + "name": "python", |
| 472 | + "nbconvert_exporter": "python", |
| 473 | + "pygments_lexer": "ipython3", |
| 474 | + "version": "3.10.17" |
423 | 475 | }
|
424 | 476 | },
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425 | 477 | "nbformat": 4,
|
|
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