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SnowCrabGenomics

Bioinformatics scripts and R scripts for snow crab RNA transcriptome assembly, whole reference genome assembly and annotation, and SNP genotyping.

Contact: nick.jeffery@dfo-mpo.gc.ca

Project Components

  1. Genome assembly using long and short read sequencing with HiC chromatin capture
  2. Genome annotation
  3. Transcriptome assembly using RNA-seq from five tissues
  4. Pool-seq of 15 snow crab populations across Atlantic Canada to examine population structure
  5. Individual whole-genome sequencing ~800 crabs for SNP genotyping
  6. Environmental association analysis

Component 4: PoolSeq

Sampling

map

Figure 1 Map of genetic sample locations overlaid on minimum winter bottom temperature based on the Bedford Institute of Oceanography North Atlantic Model (BNAM). Crab Fishing Areas (CFAs) overlaid.

Component 5: EAS

#Extractions from the Ditital Twin Ocean (DTO) - developed for the Northwest Atlantic

Figure 2 DTO extractions for minimum bottom temperature observed between 2004-2024 based the GLORYS ocean reanalysis model. Model is based on monthly average time steps.

Figure 3 DTO extractions for maximum bottom temperature observed between 2004-2024 based the GLORYS ocean reanalysis model. Model is based on monthly average time steps.

Figure 4 DTO extractions for mean bottom temperature observed between 2004-2024 based the GLORYS ocean reanalysis model. Model is based on monthly average time steps.