@@ -128,7 +128,7 @@ <h1>Differential analysis after method refinement</h1>
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Proteome Machine (GPM). You can explore their coverage by doing the following:
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</ p >
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< ul >
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- < li > On the < b > View</ b > menu, click < b > Document Grid</ b > .</ li >
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+ < li > On the < b > View</ b > menu, choose < b > Live Reports </ b > , and click < b > Document Grid</ b > (Alt-3) .</ li >
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< li > On the < b > Reports</ b > menu in the upper-left corner of the < b > Document Grid</ b > form, click
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< b > Precursors</ b > .</ li >
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< li > Scroll to the right in the < b > Document Grid</ b > to find the “Library Name” column, click it and click < b > Sort
@@ -361,7 +361,7 @@ <h2>Scheduled SRM and peak truncation</h2>
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You can also use the < b > Document Grid</ b > to create a list of all precursors with any peak truncation by doing the following:
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</ p >
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< ul >
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- < li > On the < b > View</ b > menu, click < b > Document Grid</ b > .</ li >
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+ < li > On the < b > View</ b > menu, choose < b > Live Reports </ b > , and click < b > Document Grid</ b > (Alt-3) .</ li >
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< li > On the < b > Reports</ b > menu in the < b > Document Grid</ b > view, click < b > Precursors</ b > .</ li >
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< li > On the < b > Reports</ b > menu in the < b > Document Grid</ b > view, click < b > Customize Report</ b > .</ li >
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< li > In the < b > Report name</ b > field, enter “Truncated Precursors”.</ li >
@@ -677,7 +677,7 @@ <h2>Global normalization standards</h2>
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< p >
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Now you can see that the peak area for peptide HLNGFSVPR decreases dramatically from the results acquired for the first sample to the last. The
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total peak area goes from roughly 6,000,000 to 30,000, spanning a 200-fold difference. See if you able to use the < b > Results
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- Grid</ b > (under < b > View</ b > > < b > Other Grids </ b > > < b > Results Grid</ b > ) with the HLNGFSVPR
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+ Grid</ b > (under < b > View</ b > > < b > Live Reports </ b > > < b > Results Grid</ b > ) with the HLNGFSVPR
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peptide precursor 513.7776++ selected to determine the exact maximum and minimum total peak areas. Looking at the other two standard peptides,
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you will see that they too decrease over time (VVLSGSDATLAYSAFK 2.3 to 1.1 million and AFGLSSPR 23 to 1 million). All 42 runs are supposed to
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be essentially technical replicates for these three peptides. While there is clearly systematic signal degradation for all peptides over time
@@ -1122,7 +1122,7 @@ <h2>Preparing for statistical analysis with replicate annotations</h2>
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To set the annotations you have added to the document, do the following:
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</ p >
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< ul >
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- < li > On the < b > View</ b > menu, click < b > Document Grid</ b > .</ li >
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+ < li > On the < b > View</ b > menu, choose < b > Live Reports </ b > , and click < b > Document Grid</ b > (Alt-3) .</ li >
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< li > On the < b > Reports</ b > menu, in the upper left corner of the < b > Document Grid</ b > , click < b > Replicates</ b > .
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</ li >
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</ ul >
@@ -1500,7 +1500,7 @@ <h2>Simple group comparisons in Skyline</h2>
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To inspect the group comparison you just defined do the following:
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</ p >
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< ul >
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- < li > On the < b > View</ b > menu, choose < b > Group Comparisons</ b > and click < b > Health v. Diseased</ b > .</ li >
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+ < li > On the < b > View</ b > menu, choose < b > Live Reports </ b > , then < b > Group Comparisons</ b > , and click < b > Health v. Diseased</ b > .</ li >
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</ ul >
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< p class ="keep-next ">
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Skyline will show a grid view that looks like this:
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