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Update "View > Live Reports" and "View > Libraries" references in English tutorial HTML files. (#3396)
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pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/index.html

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@@ -427,7 +427,7 @@ <h2>Configuring quantification settings:</h2>
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</ul>
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<h2>Specify the analyte concentrations of the external standards:</h2>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>In the top left of the <b>Document Grid</b>, click the <b>Reports</b> dropdown list and choose
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<b>Replicates</b>.</li>
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<li>Copy the following data and paste it into the <b>Document Grid</b> to set each of the “Standard_#” replicates <b>Sample

pwiz_tools/Skyline/Documentation/Tutorials/AuditLog/en/index.html

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@@ -75,7 +75,7 @@ <h2>Opening the audit log</h2>
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Now open the audit log by doing the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b> and click <b>Audit Log</b> (Alt + 4)</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Audit Log</b> (Alt-4)</li>
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</ul>
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<p>
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This should bring up the <b>Audit Log</b> view in its default configuration as shown below:
@@ -336,7 +336,7 @@ <h2>Specify the analyte concentrations of the external standards:</h2>
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Next, specify the analyte concentration for each replicate using the <b>Document Grid</b> as follows:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>In the top left of the <b>Document Grid</b>, click the <b>Reports</b> dropdown list and choose
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<b>Replicates</b>.</li>
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<li>In the row for FOXN1-GST, set its <b>Sample Type</b> to “Unknown”.</li>

pwiz_tools/Skyline/Documentation/Tutorials/CustomReports/en/index.html

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@@ -536,7 +536,7 @@ <h1>Quality Control Summary Reports</h1>
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To view the data for key quality control metrics follow the steps below:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>At the top of the <b>Document Grid</b> form, click the <b>Reports</b> button. A dropdown list will appear.</li>
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<li>Choose <b>Manage Reports</b> from the dropdown list.</li>
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<li>Click the import button in the <b>Manage Reports</b> form.</li>
@@ -649,7 +649,7 @@ <h1>Results Grid View</h1>
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To get started using the <b>Results Grid</b> view, do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, and click <b>Results Grid</b> (Alt-F2).</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Results Grid</b> (Alt-2).</li>
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</ul>
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<p class="keep-next">
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Skyline should now look something like this:

pwiz_tools/Skyline/Documentation/Tutorials/DDASearch/en/index.html

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@@ -362,9 +362,9 @@ <h1>Configuring Skyline to View the Imported Data</h1>
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<li>Click on that peptide’s first precursor 835.9140++ and the chromatogram for that precursor and the MS/MS spectrum for that peptide will
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appear. (Note that the bold, underlined residue “<b><u>C</u></b>” in the peptide sequence indicates a carbamidomethyl cysteine).
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</li>
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<li>If you do not see the MS/MS spectrum, on the <b>View</b> menu, click <b>Library Match</b>.</li>
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<li>If you do not see the MS/MS spectrum, on the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Match</b> (Alt-1).</li>
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<li>If you do not see as many annotated peaks in the <b>Library Match</b> view as in the image below, on the <b>View</b>
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menu, choose <b>Ion Types</b> and check <b>B</b> and <b>Y</b>.</li>
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menu, choose <b>Libraries</b>, then <b>Ion Types</b>, and check <b>B</b> and <b>Y</b>.</li>
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<li>If you do not see the entire chromatogram for the peptide, on the <b>View</b> menu, choose <b>Auto-Zoom</b> and click
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<b>None</b> (Shift-F11).</li>
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<li>Right-click the chromatogram graph, choose <b>Peptide ID Times</b>, and click <b>Aligned</b> if it is unchecked.</li>

pwiz_tools/Skyline/Documentation/Tutorials/DIA-PASEF/en/index.html

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@@ -438,7 +438,7 @@ <h1>Sample Annotation</h1>
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You are now ready to annotate the replicates you have imported:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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</ul>
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<p>
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This brings up the <b>Document Grid</b> window, which will show the <b>Proteins</b> report if you have never used it
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To perform a simple pairwise group comparison inside Skyline do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>Add</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>Add</b>.</li>
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<li>For the <b>Name</b> field, enter “By Condition”.</li>
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<li>For the <b>Control group annotation</b> field, choose “Condition”.</li>
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<li>For the <b>Control group value</b> field, choose “A”.</li>
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To see the group comparison you have just created:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>By
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>By
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Condition</b>.</li>
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</ul>
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<p>

pwiz_tools/Skyline/Documentation/Tutorials/DIA-QE/en/index.html

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@@ -443,7 +443,7 @@ <h1>Sample Annotation</h1>
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You are now ready to annotate the replicates you have imported:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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</ul>
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<p>
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This brings up the <b>Document Grid</b> window, which will show the <b>Proteins</b> report if you have never used it
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To perform a simple pairwise group comparison inside Skyline do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>Add</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>Add</b>.</li>
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<li>For the <b>Name</b> field, enter “By Condition”.</li>
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<li>For the <b>Control group annotation</b> field, choose “Condition”.</li>
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<li>For the <b>Control group value</b> field, choose “A”.</li>
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To see the group comparison you have just created:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>By
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparison</b>, and click <b>By
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Condition</b>.</li>
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</ul>
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<p>

pwiz_tools/Skyline/Documentation/Tutorials/DIA-TTOF/en/index.html

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@@ -441,7 +441,7 @@ <h1>Sample Annotation</h1>
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You are now ready to annotate the replicates you have imported:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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</ul>
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<p>
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This brings up the <b>Document Grid</b> window, which will show the <b>Proteins</b> report if you have never used it
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To perform a simple pairwise group comparison inside Skyline do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>Add</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>Add</b>.</li>
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<li>For the <b>Name</b> field, enter “By Condition”.</li>
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<li>For the <b>Control group annotation</b> field, choose “Condition”.</li>
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<li>For the <b>Control group value</b> field, choose “A”.</li>
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To see the group comparison you have just created:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Other Grids</b>, then <b>Group Comparison</b>, and click <b>By
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>By
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Condition</b>.</li>
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</ul>
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<p>

pwiz_tools/Skyline/Documentation/Tutorials/ExistingQuant/en/index.html

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@@ -310,8 +310,8 @@ <h1>Inserting a Transition List with Associated Proteins</h1>
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selections:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Ion Types</b> and click <b>B</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Charges</b> and click <b>2</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, then <b>Ion Types</b>, and click <b>B</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, then <b>Charges</b> and click <b>2</b>.</li>
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</ul>
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<p>
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You may have noticed that Skyline shows the same spectrum for both the light and heavy precursors. This spectral library may contain only
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following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>Reports</b> menu in the upper left corner of the <b>Document Grid</b>, click <b>Replicates</b>.
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</li>
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</ul>

pwiz_tools/Skyline/Documentation/Tutorials/GroupedStudies/en/index.html

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Proteome Machine (GPM). You can explore their coverage by doing the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>Reports</b> menu in the upper-left corner of the <b>Document Grid</b> form, click
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<b>Precursors</b>.</li>
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<li>Scroll to the right in the <b>Document Grid</b> to find the “Library Name” column, click it and click <b>Sort
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You can also use the <b>Document Grid</b> to create a list of all precursors with any peak truncation by doing the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>Reports</b> menu in the <b>Document Grid</b> view, click <b>Precursors</b>.</li>
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<li>On the <b>Reports</b> menu in the <b>Document Grid</b> view, click <b>Customize Report</b>.</li>
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<li>In the <b>Report name</b> field, enter “Truncated Precursors”.</li>
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<p>
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Now you can see that the peak area for peptide HLNGFSVPR decreases dramatically from the results acquired for the first sample to the last. The
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total peak area goes from roughly 6,000,000 to 30,000, spanning a 200-fold difference. See if you able to use the <b>Results
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Grid</b> (under <b>View</b> &gt; <b>Other Grids</b> &gt; <b>Results Grid</b>) with the HLNGFSVPR
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Grid</b> (under <b>View</b> &gt; <b>Live Reports</b> &gt; <b>Results Grid</b>) with the HLNGFSVPR
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peptide precursor 513.7776++ selected to determine the exact maximum and minimum total peak areas. Looking at the other two standard peptides,
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you will see that they too decrease over time (VVLSGSDATLAYSAFK 2.3 to 1.1 million and AFGLSSPR 23 to 1 million). All 42 runs are supposed to
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be essentially technical replicates for these three peptides. While there is clearly systematic signal degradation for all peptides over time
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To set the annotations you have added to the document, do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>On the <b>Reports</b> menu, in the upper left corner of the <b>Document Grid</b>, click <b>Replicates</b>.
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</li>
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</ul>
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To inspect the group comparison you just defined do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Group Comparisons</b> and click <b>Health v. Diseased</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, then <b>Group Comparisons</b>, and click <b>Health v. Diseased</b>.</li>
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</ul>
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<p class="keep-next">
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Skyline will show a grid view that looks like this:

pwiz_tools/Skyline/Documentation/Tutorials/HiResMetabolomics/en/index.html

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@@ -310,7 +310,7 @@ <h1>Importing Mass Spectrometer Runs</h1>
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<li>On the <b>View</b> menu, choose <b>Peak Areas</b> and click <b>Replicate Comparison</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Retention Times</b> and click <b>Replicate Comparison</b>.</li>
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<li>Click and drag these views to dock them above the chromatogram graphs.</li>
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<li>On the <b>View</b> menu, choose <b>Document Grid</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>In the <b>Document Grid</b> view, click the <b>Reports</b> menu, and then click <b>Molecule
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Quantification</b>.</li>
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<li>Click and drag the <b>Document Grid</b> view and dock it next to the chromatogram graphs.</li>

pwiz_tools/Skyline/Documentation/Tutorials/ImportingAssayLibraries/en/index.html

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You can also browse this newly generated spectral library in the Spectral Library Explorer by doing the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Spectral Libraries</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Explorer</b>.</li>
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</ul>
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<p>
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The spectral library was named the same as your current Skyline document, “AQUA4_Human_Blank-assay”.

pwiz_tools/Skyline/Documentation/Tutorials/LibraryExplorer/en/index.html

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To open the library explorer and view the contents of the library you just added, do the following:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Spectral Libraries</b>. </li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Explorer</b>.</li>
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</ul>
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<p>
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A message is presented about the modifications Skyline has detected in the Experiment 15N library. In your own work, you will often want to

pwiz_tools/Skyline/Documentation/Tutorials/MS1Filtering/en/index.html

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the following steps:
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</p>
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<ul>
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<li>On the <b>View</b> menu, click <b>Spectral Libraries</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Explorer</b>.</li>
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</ul>
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<p class="keep-next">
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Skyline will again offer to use the modifications it detects in the library which you chose not to add to your document
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<ul>
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<li>Click on the sequence of the first phosphopeptide K.DIDIS<b><u>S</u></b>PEFK.I and the MS/MS spectrum will appear. (Note that
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the bold, underlined residue “<b><u>S</u></b>” in the peptide sequence indicates a serine phosphorylation). </li>
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<li>If you do not see the MS/MS spectrum, on the <b>View</b> menu, click <b>Library Match</b>.</li>
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<li>If you do not see as many annotated peaks as in the image below, on the <b>View</b> menu, choose <b>Ion Types</b> and
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<li>If you do not see the MS/MS spectrum, on the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Match</b> (Alt-1).</li>
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<li>If you do not see as many annotated peaks as in the image below, on the <b>View</b> menu, choose <b>Libraries</b>, then <b>Ion Types</b> and
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check <b>A</b>, <b>B</b>, <b>Y</b> and <b>Precursor</b>.</li>
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<li>If you do not see the entire chromatogram for the peptide, on the <b>View</b> menu, choose <b>Auto-Zoom</b> and click
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<b>None</b> (Shift-F11).</li>

pwiz_tools/Skyline/Documentation/Tutorials/MethodEdit/en/index.html

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<img src="s-04.png" />
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</p>
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<ul>
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<li>On the <b>View</b> menu, choose <b>Ion Types</b>, and then click <b>B</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, then <b>Ion Types</b>, and click <b>B</b>.</li>
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</ul>
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<p>
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This makes Skyline highlight the b-ions for this peptide in purple in the spectrum graph. Here are the steps to show precursor <i>m/z</i> and

pwiz_tools/Skyline/Documentation/Tutorials/MethodRefine/en/index.html

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<ul>
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<li>Select the first peptide (YLGAYLLATLGGNASPSAQDVLK) in the <b>Targets</b> view.</li>
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<li>On the <b>View</b> menu, choose <b>Auto-Zoom</b>, and click <b>Best Peak</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Ion Types</b>, and click <b>B</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, then <b>Ion Types</b>, and click <b>B</b>.</li>
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</ul>
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<p>
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Skyline should present graphs showing both a corresponding MS/MS spectrum from a library and time-intensity chromatogram data measured for

pwiz_tools/Skyline/Documentation/Tutorials/PRMOrbitrap-PRBB/en/index.html

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</p>
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<p>
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<b>Tip!</b> For each target peptide you can view the corresponding MS2 spectrum of the library via the MS/MS Spectrum tab (usually by
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default visible, if not, go to View🡪Library Match). To select the ion type that you want to label in the MS2 spectrum right-click on the
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default visible, if not, go to View🡪Libraries🡪Library Match). To select the ion type that you want to label in the MS2 spectrum right-click on the
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spectrum and select any additional ion types you are interested in.
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</p>
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<p>
@@ -963,7 +963,7 @@ <h2>Protein quantitation using single point calibration </h2>
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Once all the data has been reviewed and properly refined, we will use Skyline to quantify the proteins of interest in our samples.
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</p>
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<ul>
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<li><b>Go</b> to “View” → “Document Grid”. </li>
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<li>On the <b>View</b> menu, choose <b>Live Reports</b>, and click <b>Document Grid</b> (Alt-3).</li>
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<li>In the Document Grid window: <b>Go</b> to “Reports” → “Peptide Ratio Results”.</li>
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</ul>
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<p>
@@ -1143,7 +1143,7 @@ <h3>Group Comparison in Skyline</h3>
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To inspect the group comparison you just defined do the following:
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</p>
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<ul>
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<li><b>“View”</b> → “Other Grids” → “Group Comparisons” → “G2M-vs-G1” or “S-vs-G1”.</li>
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<li><b>“View”</b> → “Live Reports” → “Group Comparisons” → “G2M-vs-G1” or “S-vs-G1”.</li>
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</ul>
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<p class="keep-next">
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Skyline will show a grid view that looks like this:

pwiz_tools/Skyline/Documentation/Tutorials/PRMOrbitrap/en/index.html

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Original file line numberDiff line numberDiff line change
@@ -269,7 +269,7 @@ <h2>Peptide Settings – Library tab</h2>
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</ul>
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<p>
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<b><span class="green">Tip!</span></b> You can visualize and browse all peptides of your library in the Spectral Library Explorer
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under <br /><b>View</b><b>Spectral Libraries</b>.
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under <br /><b>View</b><b>Libraries</b><b>Library Explorer</b>.
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</p>
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<p>
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<b><span class="green">Tip!</span></b> Skyline supports building libraries from many peptide spectrum matching pipeline outputs. The

pwiz_tools/Skyline/Documentation/Tutorials/SmallMoleculeIMSLibraries/en/index.html

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@@ -196,7 +196,7 @@ <h1>Adding and Exploring a Spectral Library</h1>
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To open the library explorer and view the contents of the library you just added, do the following:
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</p>
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<ul>
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<li>From the <b>View</b> menu, click <b>Spectral Libraries</b>.</li>
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<li>On the <b>View</b> menu, choose <b>Libraries</b>, and click <b>Library Explorer</b>.</li>
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</ul>
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<p class="keep-next">
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The library explorer should now look like this:

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