#Parts list
Part Description | Manufacturer | Part Number | Notes |
---|---|---|---|
Microscope config | available from FPbase | ||
Blue laser | Lasever | OEM 470nm-2W | actual wavelength ~465nm, 2W. DPSS Driver LY-II-IDA. SN 20071721 |
Green laser | Lasever | LSR532H-2W | DPSS multimode laser, 2W. DPSSL Driver FA. SN 20071501F |
Red laser | Lasever | LSR635-500 | Diode laser, 500mW. DPSSL Driver II (LSR-PS-II). SN 17111703Y |
NIR laser | Thorlabs | FPV785P | 100mW, coherence length ~50m. volume-holographic-grating (VHG) stabilized laser. SN FPV-40739 |
14-pin butterfly laser diode mount | Thorlabs | LM14S2 | |
Half-wave plate | Thorlabs | WPMH05M-780 | |
PBS | Thorlabs | PBS122 | 1/2" Polarizing Beamsplitter Cube, 620 - 1000 nm |
Aspheric collimating lens (x3) | Thorlabs | C060TMD-B | f = 9.6 mm, NA = 0.3, WD = 7.1 mm, Mounted Aspheric Lens, ARC: 600 - 1050 nm |
SMF | Thorlabs | PM780-HP | 1m long, MFD 5.3um, NA=0.12 |
Current controller | Thorlabs | LDC205C | |
Temperature controller | Thorlabs | TED200C | |
M1-M13 | Thorlabs | BB1-E02 / BB2-E02 | |
DM1 | Semrock | LM01-552-25 | Combine red and green excitation light |
Excitation filters | Semrock | LF405-488-532-635-B-OMF | |
DM2 | Semrock | LM01-480-25 | Combine blue light with red/green |
FC1 | Thorlabs | RC02FC-P01 | |
MMF | Thorlabs | M103L05 | Square core 150um x 150um, NA=0.39, multimode |
SMF | Thorlabs | P3-780PM-FC-2 | MFD 5.3um |
Aspheric collimating lens | Thorlabs | A280TM-B | f = 18.40 mm, NA = 0.15, WD = 16.88 mm, Mounted Aspheric Lens, ARC: 650 - 1050 nm. Outcouples light from fiber and directs towards DMD |
ODT beam expander lens #1 | Thorlabs | AC254-030-AB-ML | f = 30.0 mm, Ø1" Achromatic Doublet, ARC: 400 - 1100 nm |
ODT beam expander lens #2 | Thorlabs | LA1986-B-ML | N-BK7 Plano-Convex Lens, f = 125 mm, ARC: 650-1050 nm |
Fiber shaker | Custom | Design from Schroeder et al. available on GitHub | |
FC2 | Thorlabs | SM1FC | FC/PC Fiber Adapter Plate |
L1 | Thorlabs | AC254-30-A-ML | Collimate output of MMF |
Voice coil mirror | Optotune | MR-15-30 | |
L2 | Thorlabs | CLS-SL | Scan Lens with Large Field of View, 400 to 750 nm, EFL=70 mm. Imaging system between voice coil mirror and DMD lens #1 |
L3 | Thorlabs | AC508-400-A-ML | Imaging system between voice coil mirror and DMD lens #2 |
L4 | Thorlabs | AC254-075-AB-ML | Green beam expander lens #1 |
L5 | Thorlabs | AC508-500-A-ML | Green beam expander lens #2 |
Dichroic combiner | Thorlabs | FF765 | 765 nm edge BrightLine dichroic beamsplitter. Combining green and NIR before launching to DMD |
DMD | Texas Instruments | DLP Light Crafter 6500, DLP6500FYE | 1920 x 1080 mirrors, 7.56um pitch |
rotation stage | Thorlabs | PR01 | part of DMD mount |
translation stage | Thorlabs | XR25C | part of DMD mount |
z-stage | Thorlabs | MVS010 | part of DMD mount |
L6 | Nikon | MXA20696 | Nikon tube lens |
Shortpass DM | Semrock | FF750-SDi02-25x36 | 750 nm edge BrightLine short-pass dichroic beamsplitter. Separate SIM and ODT excitation light. IDEX part number FL-007128 |
ODT FT lens | Thorlabs | AC508-100-A-ML | |
ODT mirrors (3x) | Thorlabs | PF20-03-P01 | |
Fourier mask | Custom | 3D printed with Form 2, 6 ~1mm diameter holes | |
POL | Codixx | colorPol vis 500 BC4 CW01 | six element ``pizza'' polarizer |
DM3 | Chroma | zt473/532/633rdc-uf3 | All dichroic mirrors must appear in pairs to avoid degrading the polarization of the SIM beams. Matched pair mirror for DM4. Mounted on a Thorlabs WFA2002 which has been modified to attach to a kinematic mirror mount |
Shortpass DM | Semrock | FF750-SDi02-25x36 | 750 nm edge BrightLine short-pass dichroic beamsplitter. Recombine SIM and ODT excitation light. IDEX part number FL-007128 |
L7 | Thorlabs | AC508-400-A-ML | |
L8 | Thorlabs | AC508-300-A-ML | |
Microscope Stand | Thorlabs | CFB1500 | |
Cerna Arm | Thorlabs | CSA1002 | |
Dovetail adapter | Thorlabs | WFA4111 | |
Dichroic holder | Thorlabs | WFA2002 | |
DM4 | Chroma | zt473/532/633rdc-uf3 | |
EmF | Semrock | LF405-488-532-635-B-OMF | |
EmF | Chroma | ZET457NF | |
Objective adjustment arm | Thorlabs | ZFM1020 | |
OBJ | Olympus | uplfln100x | UPlanFL N 100x oil immersion, NA=1.3, FN 26.5 |
Objective piezo | Mad City Labs | Nano F-200S | |
Sample stage | Mad City Labs | MicroDrive MCL-μD1803 | |
Fluorescence detection tube lens | Olympus | SWLTU-C | |
Detection objective | Olympus | LUMPLFLN60XW | water dipping objective 60x, NA 1.0, WD 2mm, FN 26.5 |
Objective adjustment arm | Thorlabs | ZFM1020 | |
Breadboard | Thorlabs | CSA3000 | Custom machining |
ODT tube lens | Thorlabs | AC508-180-AB-ML | 4f configuration with objective |
ODT relay lens #1 | Thorlabs | AC508-100-B-ML | |
aspheric collimating lens | Thorlabs | C560TME-B | f = 13.86 mm, NA = 0.18, WD = 12.11 mm, Mounted Aspheric Lens, ARC: 650 - 1050 nm |
ODT reference arm lens #1 | Thorlabs | AC254-040-B-ML | Together with second relay lens expands the reference beam |
Imaging/reference arm combiner | Thorlabs | BBD1-E03 | |
ODT relay lens #2 | Thorlabs | AC508-300-AB-ML | |
CAM | Hamamatsu | Orca Flash4.0 v2 C11440-22CU | 1.96 ADU/e- gain, 2e- RMS readout noise, 0.71 QE at 590nm, 6.5um pixels, SN 720776 |
CAM | Phantom | VEO-1010L-72G-M | 0.39 ADU/e- gain, 10e- RMS readout noise, 0.62 QE at 650nm, 18um pixels, SN 29160 |
DAQ | National Instruments | PCIe-6343 | |
Control computer | Lenovo | ThinkStation P620 | Microsoft Windows 10 Enterprise, Ryzen Threadripper Pro 3945WX CPU (AMD) with 12 cores, 128 GB RAM |
GPU | NVIDIA | GeForce RTX 3090 | Ampere, 24GB memory |
Reconstruction computer | Custom | Ubuntu 20.04.6 LTS, X99S SLI Plus motherboard (MSI), i7-5820K CPU (Intel), 125GB RAM | |
GPU | NVIDIA | RTX A6000 | Ampere, 48GB memory |