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RNA probes are obtained by intersecting (+) strand DNA probes with exon annotations, and inverting probe strand as needed to handle +/- strand annotations, i.e. for (+) strand annotations, the (+) strand DNA probe sequences are reverse complemented to obtain the corresponding RNA-binding probe sequences. However, it appears that while probe sequences are correctly reverse complemented as needed, the value of the probe_strand field is not updated to (-) as it should be to reflect the strand inversion.
RNA probes are obtained by intersecting (+) strand DNA probes with exon annotations, and inverting probe strand as needed to handle +/- strand annotations, i.e. for (+) strand annotations, the (+) strand DNA probe sequences are reverse complemented to obtain the corresponding RNA-binding probe sequences. However, it appears that while probe sequences are correctly reverse complemented as needed, the value of the
probe_strand
field is not updated to (-) as it should be to reflect the strand inversion.Additional details: beliveau-lab/PaintSHOP_resources#2
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