Error in .rowNamesDF<-(x, value = value) : duplicate 'row.names' are not allowed

[shregeno8]

Hi this is the log from the run

── Computing Uniform Manifold Approximation and Projection (UMAP) ──

✔ UMAP was computed successfully with PCA input
✔ Successfully added 2 reducedDim slots: PCA, UMAP_PCA
✔ Pseudobulking completed (0.08 minutes taken).
✔ SingleCellExperiment successfully annotated with pseudobulk plots:
• sce@metadata$pseudobulk_rd_plots$PCA
• sce@metadata$pseudobulk_rd_plots$UMAP_PCA
• sce@metadata$pseudobulk_plots$combined_heatmap
Error in .rowNamesDF<-(x, value = value) :
duplicate 'row.names' are not allowed
Calls: annotate_merged_sce ... row.names<- -> row.names<-.data.frame -> .rowNamesDF<-
In addition: Warning message:
non-unique values when setting 'row.names': ‘NA_pNF_AACGGGATCCATTTGT-1’, ‘NA_pNF_AATTCCTTCTTGGTGA-1’,

[shregeno8]

This is my example Sample sheet

[combiz]

Hi Shri, can you try individual column values without underscores? e.g. pNF10

[shregeno8]

Hello Thanks for replying.
I tried that and same error.

[combiz]

This will need patching, but a quick fix should be to make the manifest column the first in the sample sheet.

[shregeno8]

So everything works well till this step(please see the photos which are in order)

[shregeno8]

This will need patching, but a quick fix should be to make the manifest column the first in the sample sheet.

I will try this and get back. Hoping that it works

Thanks

[shregeno8]

Hi I tried and unfortunately that doesn't work either.

[combiz]

Are you using the latest docker image (0.7.1)?

[shregeno8]

This is my example Sample sheet

Hi it worked. I had to change my group column in the sample sheet. So basically it does not accept duplicate group names. Strange because there could be cases where all the sample are from same cell lines , so don't really need to separate them by groups.

[combiz]

Thanks for filing the issue. Should be fixed in the next version!