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Using alignmentsh can cause a bgzf_read_block error in the bam file #1388

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Using alignmentsh can cause a bgzf_read_block error in the bam file#1388
@Zifeng0709

Description

@Zifeng0709
Zifeng0709
opened on Mar 19, 2025

Hello,

I’m encountering errors when processing the output of alignmentSieve with samtools sort and bgzip in a specific workflow. The error occurs only when using a filtered BAM (clean.bam) as input to alignmentSieve, but not when using the original BAM (dedup.bam). Here are the details:

### Error Workflow (Fails at samtools sort and bgzip -t)

# Step 1: Filter original BAM  
samtools view -@ ${THREADS} -F 1804 -f 2 -q 20 -b ${BOWTIE2}/sgNTC_4.dedup.bam -o ${BOWTIE2}/sgNTC_4.clean.bam  
bgzip -t ${BOWTIE2}/sgNTC_4.clean.bam  # ← Passes  
samtools index -@ 8 ${BOWTIE2}/sgNTC_4.clean.bam  

# Step 2: Run alignmentSieve on filtered BAM  
alignmentSieve -p ${THREADS} --ATACshift --blackListFileName ${BLACKLIST} \  
  -b ${BOWTIE2}/sgNTC_4.clean.bam -o ${BOWTIE2}/sgNTC_4.shift.tmp  

# Step 3: Sort and index alignmentSieve output  
samtools sort -@ ${THREADS} -O bam -o ${BOWTIE2}/sgNTC_4.shift.bam ${BOWTIE2}/sgNTC_4.shift.tmp

[E::bgzf_read] Read block operation failed with error 4 after 0 of 4 bytes
samtools sort: truncated file. Aborting

samtools index -@ ${THREADS} ${BOWTIE2}/sgNTC_4.shift.bam  

# Step 4: Validate BGZF  
bgzip -t ${BOWTIE2}/sgNTC_4.shift.tmp

[E::bgzf_read_block] Invalid BGZF header at offset 334577337
[E::bgzf_read] Read block operation failed with error 2 after 60103 of 65280 bytes

### ​Error Messages:

From samtools sort:
[E::bgzf_read] Read block operation failed with error 4 after 0 of 4 bytes
samtools sort: truncated file. Aborting
From bgzip -t:
[E::bgzf_read_block] Invalid BGZF header at offset 334577337
[E::bgzf_read] Read block operation failed with error 2 after 60103 of 65280 bytes
Error 3 in block starting at offset 334577337(13F13EB9)

### Successful Workflow

samtools index -@ 8 ${BOWTIE2}/sgNTC_4.dedup.bam  

alignmentSieve -p ${THREADS} --ATACshift --blackListFileName ${BLACKLIST} \  
  -b ${BOWTIE2}/sgNTC_4.dedup.bam -o ${BOWTIE2}/sgNTC_4.shift.tmp  

bgzip -t ${BOWTIE2}/sgNTC_4.shift.tmp  # ← Passes  

samtools sort -@ ${THREADS} -O bam -o ${BOWTIE2}/sgNTC_4.shift.bam ${BOWTIE2}/sgNTC_4.shift.tmp  
samtools index -@ ${THREADS} ${BOWTIE2}/sgNTC_4.shift.bam

Key Observations

The error occurs ​only when:
Input to alignmentSieve is ${BOWTIE2}/sgNTC_4.clean.bam (filtered via samtools view).
Both samtools sort and bgzip -t fail on the alignmentSieve output (shift.tmp).
The original BAM (dedup.bam) works flawlessly in the same pipeline.

### environment

samtools 1.18
deeptools 3.5.5
pysam 0.21.0
python 3.10.0

I asked questions in samtools' github community, see samtools/samtools#2202
The main information is pasted here

### Another infomation
In between, I tried to reuse bowtie2 to generate the original bam file and repeat the process above. This error is repeatable, and it seems that each time we use alignmentSieve to generate ${BOWTIE2}/sgNTC_4.shift.tmp, the error occurs in a different place. Details are as follows

bgzip -t ${BOWTIE2}/sgNTC_4.shift.tmp
[E::bgzf_read_block] Invalid BGZF header at offset 334577717
[E::bgzf_read] Read block operation failed with error 2 after 60105 of 65280 bytes
Error 3 in block starting at offset 334577717(13F14035)

alignmentSieve -p ${THREADS} --ATACshift --blackListFileName ${BLACKLIST} -b ${BOWTIE2}/sgNTC_4.clean.bam -o ${BOWTIE2}/sgNTC_4.shift.tmp

bgzip -t ${BOWTIE2}/sgNTC_4.shift.tmp
[E::bgzf_read_block] Invalid BGZF header at offset 334577357
[E::bgzf_read] Read block operation failed with error 2 after 60105 of 65280 bytes
Error 3 in block starting at offset 334577357(13F13ECD)

and more test

[E::bgzf_read_block] Invalid BGZF header at offset 334577337
[E::bgzf_read] Read block operation failed with error 2 after 60103 of 65280 bytes
Error 3 in block starting at offset 334577337(13F13EB9)

[E::bgzf_read_block] Invalid BGZF header at offset 334577353
[E::bgzf_read] Read block operation failed with error 2 after 59932 of 65280 bytes
Error 3 in block starting at offset 334577353(13F13EC9)

[E::bgzf_read_block] Invalid BGZF header at offset 334577402
[E::bgzf_read] Read block operation failed with error 2 after 60018 of 65280 bytes
Error 3 in block starting at offset 334577402(13F13EFA)

### Try visually inspecting the file.

Although the location of the error is different each time, it seems to be between 334577300 and 334577500

As you suggested, I used it
dd if=${BOWTIE2}/sgNTC_4.shift.tmp bs=1 skip=334577300 count=1000 | hexdump -C

The output is

00000000  18 44 ec 7d e7 00 f2 9f  ad 9e be cf b7 84 b7 ec  |.D.}............|
00000010  ab 7b 0b 99 14 9a aa a7  f0 b7 42 fc f0 10 db 25  |.{........B....%|
00000020  b7 ec f3 77 09 68 d8 4f  17 4b bc ce 01 8d c6 ff  |...w.h.O.K......|
00000030  03 53 a3 b9 31 40 93 00  00 42 43 02 00 00 00 00  |.S..1@...BC.....|
00000040  00 00 ff 06 00 42 43 02  00 1b 00 03 00 00 00 00  |.....BC.........|
00000050  00 00 00 00 00 1f 8b 08  04 00 00 00 00 00 ff 06  |................|
00000060  00 42 43 02 00 4f 37 8d  7d 0b bc 96 55 95 f7 eb  |.BC..O7.}...U...|
00000070  11 11 53 26 54 c0 73 0e  e0 a1 d4 bc 64 f9 dc 2f  |..S&T.s.....d../|
00000080  af 3a 61 79 1b e0 14 45  a5 8d a2 14 2a 4e 5e 62  |.:ay...E....*N^b|
00000090  32 ed fb 34 15 27 75 cc  b2 f8 24 4d 9b 34 46 46  |2..4.'u...$M.4FF|
000000a0  13 53 43 31 d4 54 3e 2c  48 51 13 54 f0 86 22 a4  |.SC1.T>,HQ.T..".|
000000b0  68 8a ca 31 48 d0 84 66  fd 9f 03 bc 6b ed bd ce  |h..1H..f....k...|
000000c0  7a ac 5f 87 d3 3e bf e7  bf d7 de 6b ed b5 d7 6d  |z._..>.....k...m|
000000d0  ef dd dd 68 34 06 d0 ff  7a fe f2 40 db fe 07 8c  |...h4...z..@....|
000000e0  1a b1 5d 63 66 a3 b1 a5  ed 4d 6a 9b 48 ff 8e 3d  |..]cf....Mj.H..=|
000000f0  3a 08 c2 34 6a 86 71 da  8c a2 ee b1 e9 98 b1 5f  |:..4j.q........_|
00000100  8e 9b 31 fd 9e 51 63 98  e4 59 33 2c e2 22 69 8c  |..1..Qc..Y3,."i.|
00000110  eb df 68 74 b3 8f 7b 01  c7 6f 03 44 27 33 fe f1  |..ht..{..o.D'3..|
00000120  8f 7f 7c 68 c0 51 0c f0  a8 d7 b7 02 4e da 06 f8  |..|h.Q......N...|
00000130  39 b4 19 14 86 61 5c 36  e3 20 4a a9 39 c8 e3 b2  |9....a\6. J.9...|
00000140  b1 b2 ad 05 f8 b9 6d 80  d3 b7 01 a2 93 67 0c 0a  |......m......g..|
00000150  3d c0 25 04 38 76 cb c7  eb df 78 a0 6d 3f 67 0e  |=.%.8v....x.m?g.|
00000160  ff bc e6 81 b6 0b b7 33  e6 30 0c f2 66 9c c6 65  |.......3.0..f..e|
00000170  d6 4c e2 20 6c 8c dc a9  85 f7 95 35 5b f1 5a 23  |.L. l......5[.Z#|
00000180  3e 97 da e6 8a 11 87 84  37 a6 7b f4 e7 81 97 d1  |>.......7.{.....|
00000190  ef c0 cb 82 24 6e e6 99  83 77 ee 36 bc d6 80 d1  |....$n...w.6....|
000001a0  c7 68 31 60 1b 6f 10 c3  fb f3 36 bc 16 8b 31 07  |.h1`.o....6...1.|
000001b0  17 6d 36 58 ec 8c 97 e3  7d e1 4d 7f bc 6d 6f 3d  |.m6X....}.M..mo=|
000001c0  d0 76 e4 76 c6 78 d1 41  9c a7 69 d9 cc 8b 32 10  |.v.v.x.A..i...2.|
000001d0  e3 9d fc a6 cf 8f 49 d4  76 aa 31 7f 61 94 26 cd  |......I.v.1.a.&.|
000001e0  30 89 93 a8 99 06 45 d6  58 d2 bf 85 37 e9 4d 7f  |0.....E.X...7.M.|
000001f0  bc e8 e3 1a 63 fe 5c bc  f9 0c 0f 63 73 f9 81 39  |....c.\....cs..9|
00000200  f8 dd 66 83 1f ce 78 31  7f 5b 05 fa a3 6b fd 15  |..f...x1.[...k..|
00000210  b2 33 b5 ed 65 ae e1 bc  f7 47 d1 0c d3 30 29 1a  |.3..e....G...0).|
00000220  53 d9 0a d9 79 ad bf 42  d0 c9 8b e6 1a 76 00 a7  |S...y..B.....v..|
00000230  30 c0 40 a1 f0 b3 3d 0f  b4 cd dc ce e4 48 da 24  |0.@...=......H.$|
00000240  95 90 d2 e0 93 20 8f 2a  16 6f 1b 72 8f af b6 2e  |..... .*.o.r....|
00000250  79 e7 81 b6 1d db 4c b5  95 d1 14 06 49 d8 24 61  |y.....L.....I.$a|
00000260  0c 4a 01 f8 d9 1e 7f c8  a0 7a c2 66 93 c7 92 42  |.J.......z.f...B|
00000270  ce 14 50 e3 ea 41 50 fd  de 26 53 0f 4a 0a f9 2a  |..P..AP..&S.J..*|
00000280  b9 fc 1d 5f aa 2f a5 b6  b1 96 54 87 21 f5 10 26  |..._./....T.!..&|
00000290  f4 27 82 ce 1a 83 76 68  e1 5d fa 8e 2f d5 e8 e3  |.'....vh.]../...|
000002a0  2e 4b aa 1d bc c6 0e ad  01 ff f6 1d 9f 25 77 50  |.K...........%wP|
000002b0  db c7 2c ad 15 d2 2a 49  e2 a8 a0 b1 17 41 99 34  |..,...*I.....A.4|
000002c0  26 32 a1 b9 43 99 41 74  f2 92 a5 b6 5c c0 71 0c  |&2..C.At....\.q.|
000002d0  f0 86 bf fa 52 78 3d b5  7d d5 d4 0b 11 8d 35 cc  |....Rx=.}.....5.|
000002e0  92 9c 58 12 44 61 63 36  1b f2 f5 7f f5 85 06 9d  |..X.Dac6........|
000002f0  dc 62 2a 06 07 70 06 e3  c9 37 d7 f9 9a f0 14 6a  |.b*..p...7.....j|
00000300  3b d8 5a c9 61 11 36 e3  b8 4c e9 ff 44 61 d8 68  |;.Z.a.6..L..Da.h|
00000310  f4 6b e1 9d b2 ce d7 34  e8 63 a1 b5 90 1d bc 9e  |.k.....4.c......|
00000320  ed 5b 03 fe d9 3a 9f c7  d3 a9 6d 6f 53 06 69 eb  |.[...:....moS.i.|
00000330  a4 cd 3d 81 62 28 cb bc  31 8d b1 64 fa 3a 9f c7  |..=.b(..1..d.:..|
00000340  e8 e4 05 53 08 1d 40 ae  ba 5e 57 28 5c 4d 6d a3  |...S..@..^W(\Mm.|
00000350  4d 5d 98 d2 82 2b ca 88  00 69 cb 2b 1b 3d fd 5a  |M]...+...i.+.=.Z|
00000360  80 ab 15 0a d1 c9 5c 53  17 3a 80 2b 19 60 e7 7a  |......\S.:.+.`.z|
00000370  9f c2 23 de 7d a0 ed 7e  53 75 95 34 07 29 89 0b  |..#.}..~Su.4.)..|
00000380  99 1f 71 14 0b d5 35 eb  6f be 58 df 48 6d fb 58  |..q...5.o.X.Hm.X|
00000390  06 12 b6 a7 94 66 36 6f  46 64 70 15 8d 19 6c 0e  |.....f6oFdp...l.|
000003a0  6f fc 9b 2f d6 e8 64 b9  65 20 b9 80 9c cb 0f 29  |o../..d.e .....)|
000003b0  14 8e a4 21 bf 61 8a 0d  94 4c 19 c0 32 2c f3 38  |...!.a...L..2,.8|
000003c0  68 f4 0c 68 c9 f5 b3 7f  f3 d7 c9 52 6a fb 67 73  |h..h.......Rj.gs|
000003d0  c8 51 da cc cb 9c fe 92  94 79 d8 08 d8 3a 59 fa  |.Q.......y...:Y.|
000003e0  37 7f 9d a0 8f df 9b 23                           |7......#|
1000+0 records in
1000+0 records out
000003e8
1000 bytes (1.0 kB) copied, 0.00227455 s, 440 kB/s

Add one thing

Each time dd if=${BOWTIE2}/sgNTC_4.shift.tmp bs=1 skip=334577300 count=1000 | hexdump -C gives a slightly different output

~$ dd if=${BOWTIE2}/sgKlf2_4.shift.tmp bs=1 skip=334577300 count=1000 | hexdump -C
00000000  18 44 ec 7d e7 00 f2 9f  ad 9e be cf b7 84 b7 ec  |.D.}............|
00000010  ab 7b 0b 99 14 9a aa a7  f0 b7 42 fc f0 10 db 25  |.{........B....%|
00000020  b7 ec f3 77 09 68 d8 4f  17 4b bc ce 01 8d c6 ff  |...w.h.O.K......|
00000030  03 53 a3 b9 31 40 93 00  00 42 43 02 00 00 00 00  |.S..1@...BC.....|
00000040  00 00 ff 06 00 42 43 02  00 1b 00 03 00 00 00 00  |.....BC.........|
00000050  00 00 00 00 00 1f 8b 08  04 00 00 00 00 00 ff 06  |................|
00000060  00 42 43 02 00 4f 37 8d  7d 0b bc 96 55 95 f7 eb  |.BC..O7.}...U...|
00000070  11 11 53 26 54 c0 73 0e  e0 a1 d4 bc 64 f9 dc 2f  |..S&T.s.....d../|
00000080  af 3a 61 79 1b e0 14 45  a5 8d a2 14 2a 4e 5e 62  |.:ay...E....*N^b|
00000090  32 ed fb 34 15 27 75 cc  b2 f8 24 4d 9b 34 46 46  |2..4.'u...$M.4FF|
000000a0  13 53 43 31 d4 54 3e 2c  48 51 13 54 f0 86 22 a4  |.SC1.T>,HQ.T..".|
000000b0  68 8a ca 31 48 d0 84 66  fd 9f 03 bc 6b ed bd ce  |h..1H..f....k...|
000000c0  7a ac 5f 87 d3 3e bf e7  bf d7 de 6b ed b5 d7 6d  |z._..>.....k...m|
000000d0  ef dd dd 68 34 06 d0 ff  7a fe f2 40 db fe 07 8c  |...h4...z..@....|
000000e0  1a b1 5d 63 66 a3 b1 a5  ed 4d 6a 9b 48 ff 8e 3d  |..]cf....Mj.H..=|
000000f0  3a 08 c2 34 6a 86 71 da  8c a2 ee b1 e9 98 b1 5f  |:..4j.q........_|
00000100  8e 9b 31 fd 9e 51 63 98  e4 59 33 2c e2 22 69 8c  |..1..Qc..Y3,."i.|
00000110  eb df 68 74 b3 8f 7b 01  c7 6f 03 44 27 33 fe f1  |..ht..{..o.D'3..|
00000120  8f 7f 7c 68 c0 51 0c f0  a8 d7 b7 02 4e da 06 f8  |..|h.Q......N...|
00000130  39 b4 19 14 86 61 5c 36  e3 20 4a a9 39 c8 e3 b2  |9....a\6. J.9...|
00000140  b1 b2 ad 05 f8 b9 6d 80  d3 b7 01 a2 93 67 0c 0a  |......m......g..|
00000150  3d c0 25 04 38 76 cb c7  eb df 78 a0 6d 3f 67 0e  |=.%.8v....x.m?g.|
00000160  ff bc e6 81 b6 0b b7 33  e6 30 0c f2 66 9c c6 65  |.......3.0..f..e|
00000170  d6 4c e2 20 6c 8c dc a9  85 f7 95 35 5b f1 5a 23  |.L. l......5[.Z#|
00000180  3e 97 da e6 8a 11 87 84  37 a6 7b f4 e7 81 97 d1  |>.......7.{.....|
00000190  ef c0 cb 82 24 6e e6 99  83 77 ee 36 bc d6 80 d1  |....$n...w.6....|
000001a0  c7 68 31 60 1b 6f 10 c3  fb f3 36 bc 16 8b 31 07  |.h1`.o....6...1.|
000001b0  17 6d 36 58 ec 8c 97 e3  7d e1 4d 7f bc 6d 6f 3d  |.m6X....}.M..mo=|
000001c0  d0 76 e4 76 c6 78 d1 41  9c a7 69 d9 cc 8b 32 10  |.v.v.x.A..i...2.|
000001d0  e3 9d fc a6 cf 8f 49 d4  76 aa 31 7f 61 94 26 cd  |......I.v.1.a.&.|
000001e0  30 89 93 a8 99 06 45 d6  58 d2 bf 85 37 e9 4d 7f  |0.....E.X...7.M.|
000001f0  bc e8 e3 1a 63 fe 5c bc  f9 0c 0f 63 73 f9 81 39  |....c.\....cs..9|
00000200  f8 dd 66 83 1f ce 78 31  7f 5b 05 fa a3 6b fd 15  |..f...x1.[...k..|
00000210  b2 33 b5 ed 65 ae e1 bc  f7 47 d1 0c d3 30 29 1a  |.3..e....G...0).|
00000220  53 d9 0a d9 79 ad bf 42  d0 c9 8b e6 1a 76 00 a7  |S...y..B.....v..|
00000230  30 c0 40 a1 f0 b3 3d 0f  b4 cd dc ce e4 48 da 24  |0.@...=......H.$|
00000240  95 90 d2 e0 93 20 8f 2a  16 6f 1b 72 8f af b6 2e  |..... .*.o.r....|
00000250  79 e7 81 b6 1d db 4c b5  95 d1 14 06 49 d8 24 61  |y.....L.....I.$a|
00000260  0c 4a 01 f8 d9 1e 7f c8  a0 7a c2 66 93 c7 92 42  |.J.......z.f...B|
00000270  ce 14 50 e3 ea 41 50 fd  de 26 53 0f 4a 0a f9 2a  |..P..AP..&S.J..*|
00000280  b9 fc 1d 5f aa 2f a5 b6  b1 96 54 87 21 f5 10 26  |..._./....T.!..&|
00000290  f4 27 82 ce 1a 83 76 68  e1 5d fa 8e 2f d5 e8 e3  |.'....vh.]../...|
000002a0  2e 4b aa 1d bc c6 0e ad  01 ff f6 1d 9f 25 77 50  |.K...........%wP|
000002b0  db c7 2c ad 15 d2 2a 49  e2 a8 a0 b1 17 41 99 34  |..,...*I.....A.4|
000002c0  26 32 a1 b9 43 99 41 74  f2 92 a5 b6 5c c0 71 0c  |&2..C.At....\.q.|
000002d0  f0 86 bf fa 52 78 3d b5  7d d5 d4 0b 11 8d 35 cc  |....Rx=.}.....5.|
000002e0  92 9c 58 12 44 61 63 36  1b f2 f5 7f f5 85 06 9d  |..X.Dac6........|
000002f0  dc 62 2a 06 07 70 06 e3  c9 37 d7 f9 9a f0 14 6a  |.b*..p...7.....j|
00000300  3b d8 5a c9 61 11 36 e3  b8 4c e9 ff 44 61 d8 68  |;.Z.a.6..L..Da.h|
00000310  f4 6b e1 9d b2 ce d7 34  e8 63 a1 b5 90 1d bc 9e  |.k.....4.c......|
00000320  ed 5b 03 fe d9 3a 9f c7  d3 a9 6d 6f 53 06 69 eb  |.[...:....moS.i.|
00000330  a4 cd 3d 81 62 28 cb bc  31 8d b1 64 fa 3a 9f c7  |..=.b(..1..d.:..|
00000340  e8 e4 05 53 08 1d 40 ae  ba 5e 57 28 5c 4d 6d a3  |...S..@..^W(\Mm.|
00000350  4d 5d 98 d2 82 2b ca 88  00 69 cb 2b 1b 3d fd 5a  |M]...+...i.+.=.Z|
00000360  80 ab 15 0a d1 c9 5c 53  17 3a 80 2b 19 60 e7 7a  |......\S.:.+.`.z|
00000370  9f c2 23 de 7d a0 ed 7e  53 75 95 34 07 29 89 0b  |..#.}..~Su.4.)..|
00000380  99 1f 71 14 0b d5 35 eb  6f be 58 df 48 6d fb 58  |..q...5.o.X.Hm.X|
00000390  06 12 b6 a7 94 66 36 6f  46 64 70 15 8d 19 6c 0e  |.....f6oFdp...l.|
1000+0 records in
1000+0 records out
000003a0  6f fc 9b 2f d6 e8 64 b9  65 20 b9 80 9c cb 0f 29  |o../..d.e .....)|
1000 bytes (1.0 kB) copied000003b0  14 8e a4 21 bf 61 8a 0d  94 4c 19 c0 32 2c f3 38  |...!.a...L..2,.8|
000003c0  68 f4 0c 68 c9 f5 b3 7f  f3 d7 c9 52 6a fb 67 73  |h..h.......Rj.gs|
000003d0  c8 51 da cc cb 9c fe 92  94 79 d8 08 d8 3a 59 fa  |.Q.......y...:Y.|
, 0.00186725 s, 536 kB/s
000003e0  37 7f 9d a0 8f df 9b 23                           |7......#|
000003e8

Run again

00000000  18 44 ec 7d e7 00 f2 9f  ad 9e be cf b7 84 b7 ec  |.D.}............|
00000010  ab 7b 0b 99 14 9a aa a7  f0 b7 42 fc f0 10 db 25  |.{........B....%|
00000020  b7 ec f3 77 09 68 d8 4f  17 4b bc ce 01 8d c6 ff  |...w.h.O.K......|
00000030  03 53 a3 b9 31 40 93 00  00 42 43 02 00 00 00 00  |.S..1@...BC.....|
00000040  00 00 ff 06 00 42 43 02  00 1b 00 03 00 00 00 00  |.....BC.........|
00000050  00 00 00 00 00 1f 8b 08  04 00 00 00 00 00 ff 06  |................|
00000060  00 42 43 02 00 4f 37 8d  7d 0b bc 96 55 95 f7 eb  |.BC..O7.}...U...|
00000070  11 11 53 26 54 c0 73 0e  e0 a1 d4 bc 64 f9 dc 2f  |..S&T.s.....d../|
00000080  af 3a 61 79 1b e0 14 45  a5 8d a2 14 2a 4e 5e 62  |.:ay...E....*N^b|
00000090  32 ed fb 34 15 27 75 cc  b2 f8 24 4d 9b 34 46 46  |2..4.'u...$M.4FF|
000000a0  13 53 43 31 d4 54 3e 2c  48 51 13 54 f0 86 22 a4  |.SC1.T>,HQ.T..".|
000000b0  68 8a ca 31 48 d0 84 66  fd 9f 03 bc 6b ed bd ce  |h..1H..f....k...|
000000c0  7a ac 5f 87 d3 3e bf e7  bf d7 de 6b ed b5 d7 6d  |z._..>.....k...m|
000000d0  ef dd dd 68 34 06 d0 ff  7a fe f2 40 db fe 07 8c  |...h4...z..@....|
000000e0  1a b1 5d 63 66 a3 b1 a5  ed 4d 6a 9b 48 ff 8e 3d  |..]cf....Mj.H..=|
000000f0  3a 08 c2 34 6a 86 71 da  8c a2 ee b1 e9 98 b1 5f  |:..4j.q........_|
00000100  8e 9b 31 fd 9e 51 63 98  e4 59 33 2c e2 22 69 8c  |..1..Qc..Y3,."i.|
00000110  eb df 68 74 b3 8f 7b 01  c7 6f 03 44 27 33 fe f1  |..ht..{..o.D'3..|
00000120  8f 7f 7c 68 c0 51 0c f0  a8 d7 b7 02 4e da 06 f8  |..|h.Q......N...|
00000130  39 b4 19 14 86 61 5c 36  e3 20 4a a9 39 c8 e3 b2  |9....a\6. J.9...|
00000140  b1 b2 ad 05 f8 b9 6d 80  d3 b7 01 a2 93 67 0c 0a  |......m......g..|
00000150  3d c0 25 04 38 76 cb c7  eb df 78 a0 6d 3f 67 0e  |=.%.8v....x.m?g.|
00000160  ff bc e6 81 b6 0b b7 33  e6 30 0c f2 66 9c c6 65  |.......3.0..f..e|
00000170  d6 4c e2 20 6c 8c dc a9  85 f7 95 35 5b f1 5a 23  |.L. l......5[.Z#|
1000+0 records in
1000+0 records out
00000180  3e 97 da e6 8a 11 87 84  37 a6 7b f4 e7 81 97 d1  |>.......7.{.....|
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000003e0  37 7f 9d a0 8f df 9b 23                           |7......#|
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000003e0  37 7f 9d a0 8f df 9b 23                           |7......#|
000003e8

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