Amino Acid Rules

Rules for Amino Acid Substitution

Lab Team Provides

1. For all 20 AA, what is class and what is size
2. where is the sgRNA binding domain
3. set of rules for amino acid substitution (e.g. no to proline)

Math Team Provides

1. stats on whether mutated residues always close to PAM
2. stats of whether mutated residues far from PAM are "minor mutations"

General notes/rules

• min # of mutations = 2, max # of mutations = 10 (from Kleinstiver)
• PyMol for EQR and VQR (no repack, repack, loop) <- are there any new interactions happening?
• distances for EQR/VQR? probably doesn't make sense to do until we have validated mutate_pdb