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Contact with Agriculture Industry
Steven traveled to Food & Farm Care's headquarters in Guelph to propose the CRISPR-defense for plants iGEM project to various heads of agricultural community groups. Their interest was excellent, but their worry about the nature of the virus-resistance as coming from bacteria would raise too many anti-GMO flags amongst the greater community and decrease sales of food-product overall. Steven was given the names and emails of many other groups to contact, including a plant-virus specialist from London's plant research facility. We followed up by contacting that facility and found that we'd be allowed to use their facilities to test our CRISPR-armed plant against the CAMV virus once our project was finished. I also discovered that strawberry farmers in Ontario have been plagued by a virus which we may be able to target with further development.
Project
Description
Results
Design
Requirements
Lab & Design Documentation
Measurement Interlab Study
sgRNA Swap
Target Plasmid Construction
Gibson Assembly of pCAMBIA
Math Modelling
Cauliflower Mosaic Virus (CaMV)
CaMV Spread within Arabidopsis
CRISPR/Cas9 Targeting
Modelling Viral Assembly
Modelling Viral Spread
Bioinformatics/Coding
Coding Guide
Designing sgRNA Targets for CaMV Immunity
PyMOL/PyRosetta for Windows
PyMOL/PyRosetta for Linux
PyMOL/PyRosetta for Mac
Modelling Resources
Modelling Cas9 in PyRosetta
Building PyRosetta from Source
PyRosetta Fold Tree
ABM Software Comparison
Policy & Practices
Survey Information
[Local Agriculture Outreach and Acquiring Virus Testing Facility]([Local Agriculture Outreach and Acquiring Virus Testing Facility](Local Agriculture Outreach and Acquiring Virus Testing Facility))
Teamwide Documentation
Q & A
What does this paper mean?
Outreach
Collaboration
Sponsors