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Updated README
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# Supplemental code for "Kinesin-3 responds to local microtubule dynamics to target synaptic cargo delivery to the presynapse"

[![license](https://img.shields.io/github/license/mashape/apistatus.svg?maxAge=2592000)](TODO--add_url/blob/master/LICENSE) [![DOI](https://zenodo.org/badge/doi/00.0000/zenodo.00000.svg)](http://dx.doi.org/00.0000/guedesdias.00000)
[![license](https://img.shields.io/github/license/mashape/apistatus.svg?maxAge=2592000)](TODO--add_url/blob/master/LICENSE) [![DOI](https://zenodo.org/badge/doi/10.1016/j.cub.2018.11.065/zenodo.00000.svg)](http://dx.doi.org/10.1016/j.cub.2018.11.065)

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## Contents
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**Minimal system requirements for using these scripts.** This does not include hard-drive space required for additional software packages or dependencies.

### Dependencies
The following MATLAB (R) Toolboxes are required: Image Processing Toolbox, Statistics and Machine Learning Toolbox, and Signal Processing Toolbox. Additional dependencies include [uTrack (Danuser lab)](https://www.utsouthwestern.edu/labs/danuser/software/#utrack_anc), [Bioformats](https://docs.openmicroscopy.org/bio-formats/5.9.2/users/matlab/), and MATLAB Central files where listed.
The following MATLAB (R) Toolboxes are required: Image Processing Toolbox, Statistics and Machine Learning Toolbox, and Signal Processing Toolbox. Additional dependencies include [uTrack (Danuser lab)](https://www.utsouthwestern.edu/labs/danuser/software/#utrack_anc), [Bioformats](https://docs.openmicroscopy.org/bio-formats/5.9.2/users/matlab/), and [MATLAB Central](https://www.mathworks.com/matlabcentral/) files where listed.


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Bibtex formatted reference:
```text
@article{GuedesDias2018,
Author={Guedes-Dias P, Nirschl JJ, Abreu N, Tokito MK, Magiera MM, Janke C, and Holzbaur ELF.},
Journal={Current Biology},
Title={Kinesin-3 responds to local microtubule dynamics to target synaptic cargo delivery to the presynapse},
Year{2018},
@article{Guedes-Dias2019,
abstract = {Neurons in the CNS establish thousands of en passant synapses along their axons. Robust neurotransmission depends on the replenishment of synaptic components in a spatially precise manner. Using live-cell microscopy and single-molecule reconstitution assays, we find that the delivery of synaptic vesicle precursors (SVPs) to en passant synapses in hippocampal neurons is specified by an interplay between the kinesin-3 KIF1A motor and presynaptic microtubules. Presynaptic sites are hotspots of dynamic microtubules rich in GTP-tubulin. KIF1A binds more weakly to GTP-tubulin than GDP-tubulin and competes with end-binding (EB) proteins for binding to the microtubule plus end. A disease-causing mutation within KIF1A that reduces preferential binding to GDP- versus GTP-rich microtubules disrupts SVP delivery and reduces presynaptic release upon neuronal stimulation. Thus, the localized enrichment of dynamic microtubules along the axon specifies a localized unloading zone that ensures the accurate delivery of SVPs, controlling presynaptic strength in hippocampal neurons.},
annote = {doi: 10.1016/j.cub.2018.11.065},
author = {Guedes-Dias, Pedro and Nirschl, Jeffrey J and Abreu, Nohely and Tokito, Mariko K and Janke, Carsten and Magiera, Maria M and Holzbaur, Erika L F},
doi = {10.1016/j.cub.2018.11.065},
issn = {0960-9822},
journal = {Current Biology},
month = {jan},
publisher = {Elsevier},
title = {{Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse}},
url = {https://doi.org/10.1016/j.cub.2018.11.065},
year = {2019}
}
```

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