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Cosentino, S., Sriswasdi, S., and Iwasaki, W. (2024). **SonicParanoid2: fast, accurate, and comprehensive orthology
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inference with machine learning and language models.** *Genome Biology*, **25(1)**. `https://doi.org/10.1186/s13059-024-03298-4 <https://doi.org/10.1186/s13059-024-03298-4>`_
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Derelle, R., Philippe, H., and Colbourne, J. K. (2020). **Broccoli: combining phylogenetic and network analyses for
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orthology assignment.** *Molecular Biology and Evolution*, **37(11)**.`https://doi.org/10.1093/molbev/msaa159 <https://doi.org/10.1093/molbev/msaa159>`_
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Domazet-Loso, T., Brajkovic J. and Tautz D. (2007). **A phylostratigraphy approach to uncover the genomic history of
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major adaptations in metazoan lineages.** *Trends in Genetics*, **23(11)**. `https://doi.org/10.1016/j.tig.2007.08.014 <https://doi.org/10.1016/j.tig.2007.08.014>`_
In order to extract an orthomap from `OrthoFinder <https:https://github.com/davidemms/OrthoFinder>`_ results, one needs to run `OrthoFinder <https:https://github.com/davidemms/OrthoFinder>`_.
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Mandatory OrthoFinder results files
@@ -43,9 +50,9 @@ Install OrthoFinder
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OrthoFinder installation using conda
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^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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::
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.. code-block:: bash
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conda install -c bioconda orthofinder
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conda install -c bioconda orthofinder
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Run OrthoFinder
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---------------
@@ -56,11 +63,11 @@ The species peptide file can be pre-processed e.g. to just contain the longest i
**OrthoFinder by default use diamond as the sequence search engine.** To increase sequence search sensitivity, at least use the '-S diamond_ultra_sens' option.
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To change the `'config.json' <https://raw.githubusercontent.com/davidemms/OrthoFinder/master/scripts_of/config.json>`_ and the 'diamond_ultra_sens' option from OrthoFinder, please change the 'cofig.json' as follows:
orthofinder -t 32 -a 8 -og -o last_output/ -S last -f folder_with_peptides/
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orthofinder -t 32 -a 8 -og -o last_output/ -S last -f folder_with_peptides/
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Adding a new species to an existing OrthoFinder result
@@ -151,28 +158,27 @@ ORF/CDS extraction can be done with e.g. `TransDecoder <https://github.com/Trans
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using `miniprot <https://github.com/lh3/miniprot>`_ with the "newer" annotated peptides followed by `miniprothint <https://github.com/tomasbruna/miniprothint>`_ or
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using `GALBA <https://github.com/Gaius-Augustus/GALBA>`_
Now one can use the extracted peptides with `OrthoFinder <https:https://github.com/davidemms/OrthoFinder>`_ to add them to an existing `OrthoFinder <https:https://github.com/davidemms/OrthoFinder>`_ run.
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- Place the new species peptide files in a separate folder
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::
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.. code-block:: bash
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orthofinder -t 32 -a 8 -og -S last -b last_output/Results_Sep13/WorkingDirectory/ -f new_species/
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orthofinder -t 32 -a 8 -og -S last -b last_output/Results_Sep13/WorkingDirectory/ -f new_species/
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