How to detect junction reads in 5'-scRNA-seq data?

[maozhy3]

Hello, your research is fantastic! I'm concerned about the use of junction reads as one of the bases for T-cell subcluster annotation in your study, and you say that this metric can be detected in 5'-scRNA-seq data, but I'm sorry that I wasn't able to understand your exact calculations. Would you kindly give me some introduction? Do you finished it in Seurat or CellRange?