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The LAST software was updated and it has new defaults for some of its
parameters. The alignments ran with this pipeline will not be identical to
the ones from older versions.
Added
The alignment/lastdb directory is not output anymore. It consumed space,
is not usually needed for downstream analysis, and can be re-computed
identically if needed.
The many-to-one alignment file is not output anymore by default, to save
space. To keep this file, you can run the pipeline in many-to-many mode
with the --m2m parameter.
The --seed parameter allows for all the existing values in the lastdb
program.
Errors caused by absence of alignments at training or plotting steps
are now ignored.
New parameter --export_aln_to that creates additional files containing
the alignments in a different format such as Axt, Chain, GFF or SAM.
Fixed
Incorrect detection of regions with 10 or more Ns was corrected (#18).
The --lastal_params now works as intended instead of being ignored (#22).
The workflow summary is now properly sorted at the end of the MultiQC report (#32).